Laboratory for Biophysics, Institute of Physics Belgrade

Research activities and topics


Label free erythrocytes and hemoglobin imaging

Methods:

  • TPEF: excitation 730 nm, detection 400-700 nm
  • THG (planned)
  • FCS (planned; with labelling)

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Imaging and micro processing of chitinous
structures for documents security
and entomology


Methods:

  • TPEF: excitation 730 nm, 840 nm, detection 400-700 nm, 400-450 nm
  • SHG: excitation 1040 nm, detection 520 nm; time-resolved, streak camera
  • Pattern inscription: cutting, selective photobleaching

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Patents:

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Colon tissue imaging and early stage cancer diagnostic

Methods:

  • TPEF: excitation 730 nm, detection 400-700 nm
  • SHG and polarization resolved SHG - PSHG: excitation 840 nm, detection 420 nm
  • Advanced image analysis for collagen fiber characterization (CT-FIRE, CurveAlign and FiberFit)

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Collaboration:

  • Sanja Despotović, Institute for Histology and Embryology "Aleksandar Ð. Kostić", Faculty of Medicine, University of Belgrade

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Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging

Methods:

  • TPEF: excitation 730 nm, detection 400-700 nm
  • SHG and polarization resolved SHG - PSHG: excitation 840 nm, detection 420 nm

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Design of novel fluorescent probes for biomedical imaging based on up-converting nanoparticles

Methods:

  • TPEF: excitation 730 nm (fs pulses), detection 400-700 nm
  • Photon up conversion - UC (CW): excitation 980 nm, detection 400-700 nm

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Dental tissue characterization and imaging

Methods:

  • TPEF: excitation 730 nm (dentin autoflorescence) and 820 nm (eosin fluorescence), detection 400-700 nm
  • SHG: excitation 840 nm or 1040 nm, detection 420 nm or 520 nm

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Cell surgery and electrophysiology

Methods:

  • TPEF: excitation 730 nm, detection 400-700 nm
  • THG: excitation 1040 nm, detection 347 nm
  • Pattern inscription: cutting and cell micro surgery

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Second harmonic generation in doped polymeric carbon monoxide

Methods:

  • TPEF: excitation 840 nm, detection 420 nm

Publications:


Collaboration:

  • Michael G. Pravica, Department of Physics and Astronomy, University of Nevada Las Vegas (UNLV), USA

Projects:

  • Institutional

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